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1994-10-25
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Document 3060
DOCN M94A3060
TI Monitoring of CD8/DR lymphocyte subset in pediatric HIV infection: a
preliminary study.
DT 9412
AU Tzantzoglou S; Ajassa C; Berardelli G; Falciano M; Bellagamba R; Catania
S; Infect. Dis. La Sapienza Univ., Rome, Italy.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):162 (abstract no. PB0075). Unique
Identifier : AIDSLINE ICA10/94369515
AB OBJECTIVE: to value the presence of quantitative changes of CD8/DR in
different stages of pediatric HIV infection and their significance as a
predictive marker of disease progression. METHODS: we followed the
course of CD4, CD8, CD8/DR, beta 2M, HIV-Ag in 6 children with HIV
infection by vertical trasmission (mean age: 6 years) in different
stages of infection: 4 patients (3 P2D1,1P2BD1) followed for a period of
8 months and 2 patients (1 P2A;1 P2F) for 3 months. The HIV-negative
control group consisted in 8 children (4 healthy subjects and 4 with
acute viral infections). Fresh peripheral venous blood was obtained from
each child in EDTA tubes. Three-colour lymphocyte immunophenotyping was
performed using PerCP-conjugated anti-CD4 monoclonal antibody in
combination with anti-CD8(FICT) and anti-HLA-DR (PE) monoclonal
antibodies. The resulting immunostained white blood cells were analyzed
on a FACScan, using the PAINT A GATE program. The lymphocyte gate was
defined on the scattergram. The presence of HIV-Ag and beta 2M in serum
was determined by ELISA. RESULTS: in all HIV patients we observed a
progressive decrease of CD4 and in 5 cases also an increase of CD8.
Three patients (P2A,P2F,P2BD1) showed a percentage of CD8/DR lower than
50% associated with low levels of beta 2M and absence of HIV-Ag in
serum. Three patients (P2D1) presented a percentage of CD8/DR higher
than 50% associated with high levels of beta 2M. The highest values of
CD8/DR were observed during viral acute infections (HHV6-CMV-EBV).
HIV-Ag was present in the serum of 1 patient for the whole period of
study. In another patient, HIV-Ag showed a progressive decrease until
the complete depletion, associated with a drop of CD8/DR. The percentage
of CD8/DR was 0-4% in healthy children and lower than 50% in
HIV-negative subjects with acute viral infections. DISCUSSION: Our
preliminary studies show that also in the course of pediatric HIV
infection quantitative changes of CD8/DR occurr. High levels of CD8/DR
were observed mainly in association with acute viral infections
(CMV-EBV-HHV6). The increase of CD8/DR seems to be correlated with
elevated values of beta 2M, but not always with serum HIV-Ag. However,
the monitoring of CD8/DR together with the classic surrogate markers
needs further prospective follow-up studies in order to ascertain the
immunopathogenetic and prognostic significance of this cellular subset
in pediatric HIV infection.
DE beta 2-Microglobulin/METABOLISM Antigens, CD4/BLOOD Antigens,
CD8/*BLOOD Biological Markers/BLOOD Child Female Follow-Up Studies
Human HIV Antigens/BLOOD HIV
Infections/CLASSIFICATION/*DIAGNOSIS/IMMUNOLOGY HLA-DR Antigens/*BLOOD
Immunophenotyping Leukocyte Count Male T-Lymphocyte
Subsets/*IMMUNOLOGY MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).